Amino acids

Production of 8,11‐dihydroxy fatty acids from oleic and palmitoleic acids by Escherichia coli cells expressing variant 6,8‐linoleate diol synthases from Penicillium oxalicum

10 days ago   |   By Wiley

Abstract
Recombinant Escherichia coli cells expressing 8,11-linoleate diol synthase from Penicillium chrysogenum convert oleic and palmitoleic acids to 8-hydroperoxy-9-octadecenoic acid and 8-hydroperoxy-9-hexadecenoic acid only, respectively. However, recombinant E. coli cells expressing Q889A variant 6,8-LDS from Penicillium oxalicum as an 8,11-LDS converted oleic and palmitoleic acids to 8,11-dihydroxy-9-octadecenoic acid and 8,11-dihydroxy-9-hexadecenoic acid , respectively, which were identified using LC-MS/MS analysis. To select suitable variants for producing these compounds...
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