Biodiversity

Database and primer selections affect nematode community composition under different vegetations of Changbai Mountain

7 days ago   |   By Springer

Abstract
High-throughput sequencing technology is increasingly used in the study of nematode biodiversity. However, the annotation difference of commonly used primers and reference databases on nematode community is still unclear. We compared two pairs of primers and three databases (NT v20200604, SILVA138/18s Eukaryota and PR2 v4.5 databases) on the determination of nematode community from four different vegetation types in Changbai Mountain, including mixed broadleaf-conifer forest, dark coniferous forest, betula ermanii Cham and alpine tundra. Our results showed that the selection of...
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