Bioinformatic

Screening and heterologous expression of flavone synthase and flavonol synthase to catalyze hesperetin to diosmetin

1 day ago   |   By Springer

Abstract
Objectives
In this study, 44 flavone synthases and flavonol synthases from different origins were collected. The instability index and conserved domain of the enzymes were analyzed through bioinformatics analysis, the results of which allowed us to screen suitable enzymes for constructing recombinant Escherichia coli. Defective enzymes were selected as controls.
Results
Native- and sodium dodecyl sulfate-polyacrylamide gel electrophoresis were conducted to isolate the heterologously expressed proteins. Liquid chromatography-mass spectrometry, 1 H nuclear magnetic resonance, and...
Read more ...

 


Search by Tags

   Bioinformatic      Bioinformatics      Enzymes      Cellular      Protein  


World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Cellular environments shape molecular architecture

An important cellular structure called the nuclear pore complex has larger dimensions than previously thought. A research team made this discovery using cryo-focused ion beam... Read more ...

Sr Scientist - Protein Sciences - Novartis / NIBR / GNF / San Diego...

High degree of self-motivation and attention to detail. Support the collection of analytical data. Present scientific and technical work.From Novartis - Sat, 16 Oct 2021... Read more ...

Benchmarking sequencing methods and tools that facilitate the study of...

Alternative cleavage and polyadenylation , an RNA processing event, occurs in over 70% of human protein-coding genes. APA results in mRNA transcripts with distinct 3′ ends... Read more ...

A global screening identifies chromatin-enriched RNA-binding proteins and...

Cellular RNA-binding proteins have multiple roles in post-transcriptional control, and some are shown to bind DNA. However, the global localization and the general... Read more ...

KrasG12D induces changes in chromatin territories that differentially impact...

Pancreatic ductal adenocarcinoma initiation is most frequently caused by Kras mutations. Read more ...

San Diego biotech veterans launch novel therapeutics firm targeting cancer...

Tentarix comes out of stealth mode, raises $50 million in venture funding led by Versant Ventures, Samsara BioCapital Read more ...

Genetic and process engineering strategies for enhanced recombinant...

The production of N-linked glycoproteins in genetically amenable bacterial hosts offers great potential for reduced cost, faster/simpler bioprocesses, greater customisation... Read more ...

Associate Director/Director Translational Research - Viracta Therapeutics...

5+ years of pharmaceutical or biotechnology industry experience in oncology/cancer immunotherapy. Viracta Therapeutics, a precision oncology company focused on...From Viracta... Read more ...

Long-Term Stable Reduction of Low-Density Lipoprotein in Nonhuman Primates...

Wang, L. et al. Molecular Therapy. 6, 2019-2029 (2021). Read more ...

Mitochondrial Targeted Meganuclease as a Platform to Eliminate Mutant mtDNA...

Zekonyte, U. et al. Nat Commun. 12, 3210 (2021). Read more ...