EHA101

A system for rapid gene introgression into cassava immature leaves and subsequent recovery of transformed lines

21 days ago   |   By Springer

Abstract
Friable embryogenic callus currently forms the tissue of choice for gene transfer to cassava tissues. However, this technology has not been sufficiently adopted in most laboratories, partly because of the challenging protocols involved in FEC production. Therefore, the development of transformation systems targeting readily accessible explants could enable the rapid improvement of this essential root tuber. This study developed a simple, efficient, and rapid transformation protocol for three elite cassava cultivars using immature leaf lobes. Factors affecting callus and somatic...
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