Plants

Precision genome editing in plants via gene targeting and subsequent break‐induced single‐strand annealing

9 days ago   |   By Wiley

Summary
Genome editing via artificial nucleases such as CRISPR/Cas9 has become popular in plants now. However, small insertions or deletions are major mutations and nucleotide substitutions rarely occur when DNA cleavage is induced. To induce nucleotide substitutions, a base editor utilizing dead or nickase‐type Cas9 fused with deaminase have been developed. However, the direction and position of practical substitution are still limited. In this context, homologous recombination ‐mediated gene targeting has advantages because any mutations existing on the donor DNA are copied and passed...
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CRISPR/Cas‐based precision genome editing via microhomology‐mediated end joining

9 days ago   |   By Wiley

Summary
Gene editing and/or allele introgression with absolute precision and control appear to be the ultimate goals of genetic engineering. Precision genome editing in plants has been developed through various approaches, including oligonucleotide‐directed mutagenesis , base editing, prime editing, and especially homologous recombination ‐based gene targeting. With the advent of CRISPR/Cas for the targeted generation of DNA breaks or double‐stranded breaks ), a substantial advancement in HR‐mediated precise editing frequencies has been achieved. Nonetheless, further research needs to be...
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