Protein

Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii

2 days ago   |   By Wiley

Abstract
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe by the Food and Drug Administration. Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product...
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Retrospective Assessment of Clonal Origin of Cell Lines

2 days ago   |   By Wiley

Abstract
Cell lines used for the manufacture of recombinant proteins are expected to arise from a single cell as a control strategy to limit variability and ensure consistent protein production. Health authorities require a minimum of two rounds of limiting dilution cloning or its equivalent to meet the requirement of single cell origin. However, many legacy cell lines may not have been generated with process meeting this criteria potentially impeding the path to commercialization. A general monoclonality assessment strategy was developed based on using the site of plasmid integration for...
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Culturing Chinese hamster ovary cells on Cyclo Olefin Polymer triggers epithelial‐mesenchymal transition and spheroid formation, which increases the foreign gene expression driven by the MMLV‐LTR promoter

2 days ago   |   By Wiley

Abstract
Chinese hamster ovary cells are frequently used for recombinant protein production as a host. While the RPP has been proven successful, there is still a compelling need for further improvement. Cyclo Olefin Polymer is a plastic material widely utilized due to its properties including its low protein absorption. We applied this as a raw material for RPP cell culture to see if the COP is suitable. A recombinant CHO cell line expressing the human erythropoietin gene under the control of the MMTV‐LTR was established. When the cells were cultured in a dish made from COP, the cells...
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Impact of iron raw materials and their impurities on CHO metabolism and recombinant protein product quality

2 days ago   |   By Wiley

Abstract
Cell culture medium composition affects cell growth and critical quality attributes of monoclonal antibodies and recombinant proteins. One essential compound needed within the medium is iron because of its central role in many cellular processes. However, iron is also participating in Fenton chemistry leading to the formation of reactive oxygen species causing cellular damage. Therefore, this study sought to investigate the impact of iron in CCM on Chinese hamster ovary cell line performance, and CQAs of different recombinant proteins. Addition of either ferric ammonium citrate or...
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World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Meet the Alumni Award Honorees 2021

Meet the Alumni Award Honorees 2021

For 42 years, UC San Diego's Alumni Awards have celebrated the distinguished work and personal accomplishments of Tritons who bring honor and distinction to our alumni ranks... Read more ...

‘Sunny Days' Student Festival on the Horizon

‘Sunny Days' Student Festival on the Horizon

There have been too many dark clouds hovering over the past year during the global pandemic. To inspire a renewed sense of fun and excitement, Associated Students Concerts and... Read more ...

Nurses By Day, Social Justice Activists By Night

Nurses By Day, Social Justice Activists By Night

Since late May 2020, a team of registered nurses in San Diego has taken part in efforts advocating for racial equity and social justice. After working hospital shifts, they... Read more ...

A cancer vaccine built from stem cells? Stanford candidate shows promise in...

A cancer vaccine built from stem cells? Stanford candidate shows promise in pancreatic tumors aliu Thu, 05/06/2021 - 06:08 Read more ...

A molecular trap inside microtubules probes luminal access by soluble proteins

Nature Chemical Biology, Published online: 03 May 2021; doi:10.1038/s41589-021-00791-w A chemically inducible technique for trapping cytosolic proteins in microtubules in cells... Read more ...

Susan Bukata, MD, Named Chair of the Department of Orthopaedic Surgery

Bukata is only the fourth woman in the U.S. to lead a health system's orthopedic surgery department. Read more ...

A trait of the rare few whose bodies naturally control HIV: 'trained' immune...

Scientists have discover that 'elite controllers' have myeloid dendritic cells that display characteristics of trained innate immune cells. Read more ...

How mitochondria make the cut

With the help of their custom-built super-resolution microscope, biophysicists have discovered where and why mitochondria divide, putting to rest controversy about the... Read more ...

Sustained delivery of MMP-9 siRNA via thermosensitive hydrogel accelerates...

Excessive expression of matrix metalloproteinase 9 impedes healing of diabetic chronic wounds, thus wound dressing that could effectively inhibit the expression of MMP-9 offers... Read more ...

The unpredictable carbon nanotube biocorona and a functionalization method...

The intrinsic physicochemical properties of carbon nanotubes make them unique tools in nanotechnology. Their elemental composition, resilience, thermal properties, and surface... Read more ...