Protein

Semicontinuous system for the production of recombinant mCherry protein in Chlamydomonas reinhardtii

1 day ago   |   By Wiley

Abstract
Biotechnology advances have allowed bacteria, yeasts, plants, mammalian and insect cells to function as heterologous protein expression systems. Recently, microalgae have gained attention as an innovative platform for recombinant protein production, due to low culture media cost, compared to traditional systems, as well as the fact that microalgae such as Chlamydomonas reinhardtii are considered safe by the Food and Drug Administration. Previous studies showed that recombinant protein production in traditional platforms by semicontinuous process increased biomass and bio product...
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Retrospective Assessment of Clonal Origin of Cell Lines

1 day ago   |   By Wiley

Abstract
Cell lines used for the manufacture of recombinant proteins are expected to arise from a single cell as a control strategy to limit variability and ensure consistent protein production. Health authorities require a minimum of two rounds of limiting dilution cloning or its equivalent to meet the requirement of single cell origin. However, many legacy cell lines may not have been generated with process meeting this criteria potentially impeding the path to commercialization. A general monoclonality assessment strategy was developed based on using the site of plasmid integration for...
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Culturing Chinese hamster ovary cells on Cyclo Olefin Polymer triggers epithelial‐mesenchymal transition and spheroid formation, which increases the foreign gene expression driven by the MMLV‐LTR promoter

1 day ago   |   By Wiley

Abstract
Chinese hamster ovary cells are frequently used for recombinant protein production as a host. While the RPP has been proven successful, there is still a compelling need for further improvement. Cyclo Olefin Polymer is a plastic material widely utilized due to its properties including its low protein absorption. We applied this as a raw material for RPP cell culture to see if the COP is suitable. A recombinant CHO cell line expressing the human erythropoietin gene under the control of the MMTV‐LTR was established. When the cells were cultured in a dish made from COP, the cells...
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Impact of iron raw materials and their impurities on CHO metabolism and recombinant protein product quality

1 day ago   |   By Wiley

Abstract
Cell culture medium composition affects cell growth and critical quality attributes of monoclonal antibodies and recombinant proteins. One essential compound needed within the medium is iron because of its central role in many cellular processes. However, iron is also participating in Fenton chemistry leading to the formation of reactive oxygen species causing cellular damage. Therefore, this study sought to investigate the impact of iron in CCM on Chinese hamster ovary cell line performance, and CQAs of different recombinant proteins. Addition of either ferric ammonium citrate or...
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World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Resident Immune Cells Nurse Brain Cells after Seizure

Resident Immune Cells Nurse Brain Cells after Seizure

Mayo Clinic researchers have found evidence that a type of resident immune cell in the brain helps protect neurons after seizures. Their results, published in Cell Reports... Read more ...

Macrophage Communication is Key During Viral Infection

Macrophage Communication is Key During Viral Infection

A new finding from Mayo Clinic researchers and colleagues may suggest treatment options for severe viral infections and new avenues for research into tissue regeneration after... Read more ...

Scientist - Sigma Inc - La Jolla, CA

20710-1 Scientist La Jolla, CA 3 Years*. Sigma Inc is seeking a Bench Scientist to work onsite at its clients location in La Jolla, CA. $26.00 - $31.50 an hourFrom Indeed... Read more ...

Expelling cancer by squeezing out rogue cells

Expelling cancer by squeezing out rogue cells aliu Wed, 05/05/2021 - 08:01 Read more ...

CVS now offering walk-in coronavirus vaccination at pharmacies in San Diego...

The pharmacy chain is also offering same-day appointment scheduling Read more ...

Cymbiotika Introduces Rya Organics for Its Market-leading CBD Products

SAN DIEGO-- CBD-Cymbiotika, a leading nutritional supplement brand known for creating pure, clinically backed supplements, announces the launch of Rya Organics. Read more ...

Synthesis, biological evaluation and docking studies of 1,2,4-oxadiazole...

1,2,4-oxadiazole derivatives exhibited significant anti-cancer activity when they were evaluated, against human cancer cell lines. They also showed anti-inflammatory... Read more ...

Manager, Site Operations | Astellas

Job Description Developing innovative therapies is one of the most challenging, most essential and personally rewarding fields in science. This is the most exciting time to be... Read more ...

The Feasibility of 12-Lead Computational Analysis in Patients after COVID-19...

CARLSBAD, Calif.-- arrhythmias-vMap™ analysis was able to provide arrhythmia insights beyond those available from a standard 12-lead ECG in patients following COVID-19 infection. Read more ...

3rd Shift QC Microbiology Scientist - Eurofins - Oceanside, CA

Excellent communication and attention to detail. Calculate, evaluate, review, and properly communicate data verbally and in written format.From Eurofins - Wed, 05 May 2021... Read more ...