Microbial Cell Factories

EXPRESSION OF THE HUMAN MOLECULAR CHAPERONE DOMAIN BRI2 BRICHOS ON A GRAM PER LITER SCALE WITH AN E. COLI FED-BATCH CULTURE

The human Bri2 BRICHOS domain inhibits amyloid formation and toxicity and could be used as a therapeutic agent against amyloid diseases....

FINE-TUNING THE EXPRESSION OF PATHWAY GENE IN YEAST USING A REGULATORY LIBRARY FORMED BY FUSING A SYNTHETIC MINIMAL PROMOTER WITH DIFFERENT KOZAK VARIANTS

Tailoring gene expression to balance metabolic fluxes is critical for the overproduction of metabolites in yeast hosts, and its implementation...

ENFORCING ATP HYDROLYSIS ENHANCED ANAEROBIC GLYCOLYSIS AND PROMOTED SOLVENT PRODUCTION IN CLOSTRIDIUM ACETOBUTYLICUM

The intracellular ATP level is an indicator of cellular energy state and plays a critical role in regulating cellular metabolism. Depletion...

ENGINEERING OF SACCHAROMYCES CEREVISIAE FOR THE ACCUMULATION OF HIGH AMOUNTS OF TRIACYLGLYCEROL

Fatty acid-based substances play an important role in many products, from food supplements to pharmaceutical products and biofuels....

PRODUCTION OF TRANS-CINNAMIC ACID BY WHOLE-CELL BIOCONVERSION FROM L-PHENYLALANINE IN ENGINEERED CORYNEBACTERIUM GLUTAMICUM

Trans-cinnamic acid is a phenylpropanoid with a broad spectrum of biological activities including antioxidant and antibacterial activities,...

A CELL ENGINEERING APPROACH TO ENZYME-BASED FED-BATCH FERMENTATION

A fundamental problem associated with E. coli fermentations is the difficulty in achieving high cell densities in batch cultures, attributed...

PROTEOMIC ANALYSIS REVEALED THE ROLES OF YRR1 DELETION IN ENHANCING THE VANILLIN RESISTANCE OF SACCHAROMYCES CEREVISIAE

Vanillin is one of the important phenolic inhibitors in Saccharomyces cerevisiae for bioconversion of lignocellulosic materials and...

DESIGN AND HETEROLOGOUS EXPRESSION OF A NOVEL DIMERIC LL37 VARIANT IN PICHIA PASTORIS

The antimicrobial peptide LL37 is produced by white blood cells and various epithelial cells, and has the outstanding advantages of...

THE SIGNALING ROLE OF EXTRACELLULAR ATP IN CO-CULTURE OF SHIRAIA SP. S9 AND PSEUDOMONAS FULVA SB1 FOR ENHANCING HYPOCRELLIN A PRODUCTION

Adenosine 5′-triphosphate plays both a central role as an intracellular energy source, and a crucial extracellular signaling role in...

CORRECTION TO: GOLD STANDARD FOR NUTRITION: A REVIEW OF HUMAN MILK OLIGOSACCHARIDE AND ITS EFFECTS ON INFANT GUT MICROBIOTA

EFFECTS OF ACUC ON THE GROWTH DEVELOPMENT AND SPINOSAD BIOSYNTHESIS OF SACCHAROPOLYSPORA SPINOSA

Acetoin utilization protein is a type I histone deacetylase which is highly conserved in bacteria. The acuC gene is related to the...

CELLULOSE INDUCED PROTEIN 1 FROM TRICHODERMA REESEI ENHANCES THE ENZYMATIC HYDROLYSIS OF PRETREATED LIGNOCELLULOSE

Trichoderma reesei is currently the main strain for the commercial production of cellulase. Cellulose induced protein 1 is one of the...

ALTERNATIVE OXIDASE GENE INDUCED BY NITRIC OXIDE IS INVOLVED IN THE REGULATION OF ROS AND ENHANCES THE RESISTANCE OF PLEUROTUS OSTREATUS TO HEAT STRESS

In China, during the cultivation process of Pleurotus ostreatus, the yield and quality of fruiting bodies are easily affected by high...

CO-CULTURING EXPERIMENTS REVEAL THE UPTAKE OF MYO-INOSITOL PHOSPHATE SYNTHASE IN AN INOSITOL AUXOTROPH OF SACCHAROMYCES CEREVISIAE

Myo-Inositol Phosphate Synthase catalyzes the conversion of glucose 6- phosphate into inositol phosphate, an essential nutrient and...

FUNCTIONAL ANALYSIS OF MULTIPLE NIFB GENES OF PAENIBACILLUS STRAINS IN SYNTHESIS OF MO-, FE- AND V-NITROGENASES

Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively....

IMPACT OF ORGANIC CARBON ACQUISITION ON GROWTH AND FUNCTIONAL BIOMOLECULE PRODUCTION IN DIATOMS

Diatoms are unicellular photosynthetic protists which constitute one of the most successful microalgae contributing enormously to global...

EXPRESSION OF ANTIBODY FRAGMENTS IN SACCHAROMYCES CEREVISIAE STRAINS EVOLVED FOR ENHANCED PROTEIN SECRETION

Monoclonal antibodies, antibody fragments and fusion proteins derived thereof have revolutionized the practice of medicine. Major challenges...

BIODEGRADATION OF FLONICAMID BY ENSIFER ADHAERENS CGMCC 6315 AND ENZYMATIC CHARACTERIZATION OF THE NITRILE HYDRATASES INVOLVED

Flonicamid (N-cyanomethyl-4-trifluoromethylnicotinamide, FLO) is a new type of pyridinamide insecticide that regulates insect growth....

AUGMENTED PEROXISOMAL ROS BUFFERING CAPACITY RENDERS OXIDATIVE AND THERMAL STRESS CROSS-TOLERANCE IN YEAST

Thermotolerant yeast has outstanding potential in industrial applications. Komagataella phaffii is a common cell factory for industrial...

SYSTEMATIC IDENTIFICATION OF A PANEL OF STRONG PROMOTER REGIONS FROM LISTERIA MONOCYTOGENES FOR FINE-TUNING GENE EXPRESSION

Attenuated Listeria monocytogenes has been widely used as a vaccine vector in the prevention and treatment of pathogen infection and...