Virus

Quality Control Testing, Characterization and Critical Quality Attributes of Adeno‐Associated Virus Vectors Used for Human Gene Therapy

3 days ago   |   By Wiley

This article describes quality control testing and characterization of adeno‐associated virus gene therapy vectors for clinical product development. It explores the relationship between AAV vector design and manufacturing process design with product critical quality attributes required for safety and durable efficacious expression, with emphasis on vector immunogenicity.
Abstract
For adeno‐associated virus ‐based human gene therapy, challenges for the translation of promising research results to successful clinical development include optimization of vector design and manufacturing...
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Evaluation of the Single‐Use Fixed‐Bed Bioreactors in Scalable Virus Production

Evaluation of the Single‐Use Fixed‐Bed Bioreactors in Scalable Virus Production

3 days ago   |   By Wiley

Adherent cells can attach and grow in three‐dimensional fixed‐bed matrix. The single use disposable upstream technology has been implemented in many different viral vector and vaccine productions. Main process steps in viral production contain cell expansion phase, induction of the virus production and harvest.
Abstract
The accelerating development of gene therapy from research towards clinical trials and beyond has elevated the demand for practical viral vector‐manufacturing solutions. The use of disposable upstream technology is gaining traction in clinical manufacturing. Packed‐bed or...
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Outside Front Cover:

Outside Front Cover:

3 days ago   |   By Wiley

Stable cell lines provide scalable and high yielding production systems enabling the development of efficient manufacturing bioprocesses, essential for the supply of viral vectors for gene and cell therapies. The establishment of lentiviral vector producer cell lines is however very challenging. In this review, the major bottlenecks and achievements in the development of lentiviral vector stable cell lines are discussed. This is reported by Mariana V. Ferreira, Elisa T. Cabral, Ana Sofia Coroadinha in the article 2000017 .
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Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production

Homologous Recombination Offers Advantages over Transposition‐Based Systems to Generate Recombinant Baculovirus for Adeno‐Associated Viral Vector Production

3 days ago   |   By Wiley

The baculovirus/insect cell is a standard platform for manufacturing of adeno‐associated viral vectors. This study compares homologous recombination and transposition to generate the baculovirus expressing vectors. Both methods allow generation of high titers of AAV vectors, but BEVs generated by HR are genetically more stable and devoid of any bacterial DNA sequences.
Abstract
Viral vectors have a great potential for gene delivery, but manufacturing is a big challenge for the industry. The baculovirus‐insect cell is one of the most scalable platforms to produce recombinant...
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Separating Empty and Full Recombinant Adeno‐Associated Virus Particles Using Isocratic Anion Exchange Chromatography

Separating Empty and Full Recombinant Adeno‐Associated Virus Particles Using Isocratic Anion Exchange Chromatography

3 days ago   |   By Wiley

Robust and scalable separation of empty particles from recombinant adeno‐associated virus vectors is key in rAAV production. This separation is currently achieved via linear gradient elution chromatography which is challenging to implement at large scale. Improved separation resolution is demonstrated between the empty and full particles by combining NaCl and MgCl 2 as eluents, enabling an isocratic anion exchange chromatography separation.
Abstract
The development of recombinant adeno‐associated virus gene therapies is becoming an increasing priority in the biotherapeutic landscape. One...
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Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production

Production of Modified Vaccinia Ankara Virus by Intensified Cell Cultures: A Comparison of Platform Technologies for Viral Vector Production

3 days ago   |   By Wiley

Modified Vaccinia Ankara virus is a viral vector used for gene therapies. Here, MVA virus is produced with AGE1.CR.pIX suspension cells using different platform technologies. The authors then compared and discussed the pros and cons between the different platform technologies, based on the virus production yields and other criteria such as scalability and process robustness.
Abstract
Modified Vaccinia Ankara virus is a promising vector for vaccination against various challenging pathogens or the treatment of some types of cancers, requiring a high amount of virions per dose for vaccination...
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World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Primer on Carbon Dioxide Removal Provides Vital Resource at Critical Time

Primer on Carbon Dioxide Removal Provides Vital Resource at Critical Time

Berkeley Lab researchers are working on ways to sequester more carbon in soil, including through agricultural practices. Scientists say that any serious plan to address... Read more ...

A 1-Atom-Deep Look at a Water-Splitting Catalyst

A 1-Atom-Deep Look at a Water-Splitting Catalyst

This illustration shows two possible types of surface layers for a catalyst that performs the water-splitting reaction, the first step in making hydrogen fuel: The gray surface... Read more ...

New Research Technique Sheds Light on Least Understood Part of Lithium Batteries

New Research Technique Sheds Light on Least Understood Part of Lithium Batteries

A scientist works on the MALDI (matrix-assisted laser desorption/ionization) mass spectrometer at Berkeley Lab's Molecular Foundry. One of the aspects of lithium-ion batteries... Read more ...

Ozone Generators May Help Remediate Contamination Caused by Thirdhand Smoke

Ozone Generators May Help Remediate Contamination Caused by Thirdhand Smoke

Residue from cigarettes can linger on indoor surfaces for years and even decades. In 10 years of studying thirdhand smoke, which is the toxic cigarette residue that clings to... Read more ...

Financial Times: Get vaccine new variant ready

Financial Times: Get vaccine new variant ready

GreenLight Biosciences explain to the Financial Times how messenger RNA can help get the Covid-19 vaccine new variant ready. GreenLight Biosciences spoke to the Financial Times... Read more ...

Shine On: Avalanching Nanoparticles Break Barriers to Imaging Cells in Real Time

Shine On: Avalanching Nanoparticles Break Barriers to Imaging Cells in Real Time

From left: Experimental images of thulium-doped avalanching nanoparticles separated by 300 nanometers; at right, simulations of the same material. (Credit: Berkeley Lab and... Read more ...

Protecting the genome from transposon activation

Transposons are foreign DNA elements capable of random insertion into the genome, an event that can be very dangerous for a cell. Their activity must be silenced to maintain... Read more ...

Landos Biopharma guns for a $100 million IPO to boost AI autoimmune R&D work

Landos Biopharma guns for a $100 million IPO to boost AI autoimmune R&D work badams Fri, 01/15/2021 - 10:03 Read more ...

The Use of the Rare TTA Codon in Streptomyces Genes: Significance of the...

Abstract Streptomycetes, Gram-positive bacteria with huge and GC-rich genomes provide an ample example of codon usage bias taken to the extreme. Particularly, in all sequenced... Read more ...

Pathogenic Microbial Profile and Antibiotic Resistance Associated with...

Abstract Phenotyping based on conventional microbiological, physiological, and molecular analysis by using ARDRA technique was developed with the aim to assess the pathogenic... Read more ...