Enzymes

Production, purification and physicochemical characterization of D-xylose/glucose isomerase from Escherichia coli strain BL21

19 days ago   |   By Springer

Abstract
Cell lysate of Escherichia coli strain BL21 showed significant D-glucose isomerase activity. The rate of glucose conversion was increased up to 40% when cells were induced with 1% D-xylose. E. coli BL21 xylose isomerase was purified to homogeneity, up to 1.9-fold with overall 10.88% enzyme yield by heat shock, salting out and electro-elution. The molecular mass of ECXI-BL21 was estimated as 43.9 kDa on SDS-PAGE. pH opt. and T opt. of the enzyme were calculated as 7.0 and 50 °C, respectively. Activation energy of ECXI-BL21 was 45 kJ/mol. Enzyme was stable from 30 to 55 °C and at pH...
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