Genetics

Rapid generation and selection of Cas9-engineering TRP53 R172P mice that do not have off-target effects

9 days ago   |   By BMC Biotechnology

Genetic mutations cause severe human diseases, and suitable animal models to study the regulatory mechanisms involved are required. The CRISPR/Cas9 system is a powerful, highly efficient and easily manipulated...
Read more ...

 

Sustained transgene expression from sleeping beauty DNA transposons containing a core fragment of the HNRPA2B1-CBX3 ubiquitous chromatin opening element

9 days ago   |   By BMC Biotechnology

DNA transposon-based vectors are effective nonviral tools for gene therapy and genetic engineering of cells. However, promoter DNA methylation and a near-random integration profile, which can result in transge...
Read more ...

 

Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice

9 days ago   |   By Wiley

Abstract
Recently, some newly engineered SpCas9 variants, such as xCas9 3.7 and Cas9‐NG, expand the PAM recognition site to NG and show function efficiency in mammalian cells. However, there is still not enough comparative data for their efficiencies on the genome and base editing in plants. In addition, there is also a lack of enhanced specific Cas9 variant with NG PAM. Here, we developed a serial of genome editing tools using different Cas9 variants containing the xCas9, the Cas9‐NG and the enhanced specific Cas9‐NG , and of cytosine base editors (CBE 4 editors, xCas9n‐CBE, Cas9n‐NG‐CBE...
Read more ...

 


Search by Tags

   Genetics      Animals      Engineering      Research article      Genes      Gene      Transgenic      Therapies      DNA      Gene Therapy      Genetic engineering      Plants      Nucleotide      Genome      Nucleotides      Genomics  


World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

World Biotechnology Congress 2019

Hilaris Conferences is going to host its premier World Biotechnology Congress 2019 during October 01-02, 2019, Valencia, Spain. Biotechnology 2019 Congress aim to create a... Read more ...

2nd International Conference on Tissue Science and Regenerative Medicine

This conference is designed for experts in academia and industries working in the tissue science and regenerative field, this conference will examine cutting-edge research in... Read more ...

Cover Image, Volume 87, Issue 12

Cover Image, Volume 87, Issue 12

Reverse Cover: The cover image is based on the Review Critical assessment of methods of protein structure prediction ‐Round XIII by Andriy Kryshtafovych, Torsten Schwede, Maya... Read more ...

With “Inflection Year” Ahead, Ovid Adds Novartis's Tardio to C-Suite

With “Inflection Year” Ahead, Ovid Adds Novartis's Tardio to C-Suite

About five years ago, former Teva Pharmaceutical CEO Jeremy Levin launched Ovid Therapeutics with a plan to develop treatments for rare brain diseases. Now, with the completion... Read more ...

Underdog Launches With $4M to Target “Toxic” Form of Cholesterol

Underdog Launches With $4M to Target “Toxic” Form of Cholesterol

Underdog Pharmaceuticals has launched with nearly $4 million in seed funding to develop treatments for atherosclerosis, a progressive condition in which arteries become clogged... Read more ...

Alkermes Eyes Brain Disorders and Beyond With $100M Deal for Rodin

Alkermes Eyes Brain Disorders and Beyond With $100M Deal for Rodin

Rodin Therapeutics has limited data from human studies about its neurodegeneration drug, but the company's new approach to treating brain disorders has proven attractive enough... Read more ...

Yelick Recognized for Outstanding Leadership in HPC

Yelick Recognized for Outstanding Leadership in HPC

Katherine Yelick has been honored by HPCwire as their Editor's Choice for Outstanding Leadership in high-performance computing. (Photo courtesy of Berkeley Lab Computing... Read more ...

Patient Storytelling: There's an Art to it!

Patient Storytelling: There's an Art to it!

After 30-plus years working in Washington DC, it's clear healthcare decision makers recognize that a patient's story can add value to a discussion when data cannot. While the... Read more ...

Synthetic control devices for gene regulation in Penicillium chrysogenum

Orthogonal, synthetic control devices were developed for Penicillium chrysogenum, a model filamentous fungus and industrially relevant cell factory. In the synthetic... Read more ...

Substantially improving the enantioconvergence of PvEH1, a Phaseolus...

Epoxide hydrolase can regioselectively catalyze the oxirane ring-opening hydrolysis of rac-epoxides producing the corresponding chiral diols. In our laboratory, a gene named... Read more ...

Growth of the facultative chemolithoautotroph Ralstonia eutropha on organic...

The chemolithoautotrophic β-proteobacterium Ralstonia eutropha H16 is one of the most studied model organisms for growth on H2 and CO2. R. eutropha H16 is also a biologically... Read more ...

Temporary Customer Service Rep. - SeaSon - Carlsbad, CA

Critical thinker with strong attention to detail, and ability to problem solve and establish solutions in a high-growth and fast-pace environment.From Season - Tue, 19 Nov 2019... Read more ...